Advances in Giardia Research by Peter Malcolm Wallis, Brian R. Hammond

By Peter Malcolm Wallis, Brian R. Hammond

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X37,000. numbers of symbionts were seen.  There is no evidence to suggest that these vacuoles are involved in the uptake of particles as large as bacteria.  We consider it likely that the intracellular bacteria we observed in this study are symbiotic because of their intact morphological appearance and the absence of any sign of intracellular digestion.  It seems likely that this relationship was the result of the parasite being overwhelmed by large numbers of bacteria capable of participating in the relationship.

Future studies on the localization of the parasite enzymes on the plasma membrane and the molecular mechanisms of the Page 36 Figure 3.  Branched filamentous processes and rufflings on the surface can be seen clearly (original magnification × 1250) with the surface of fibroblasts (original magnification × 1250).  This simple in vitro assay system is rapid and sensitive compared with in vivo systems for the study of pathogenesis. Acknowledgements This work was supported by a research grant (70/288/85­ECD II) from the Indian Council of Medical Research.

Gastroenterology, In press. 9­21.  lamblia trophozoites.  These observations suggest that direct cell to parasite contact may play an important role in damaging the host cells.  One reported the significance of bile salt deconjugation by the parasite (12) and the other the cytopathogenic effects of Giardia on cell monolayers, in vitro (11).  lamblia on HeLa and Vero cells.  30888 (9) were maintained in Diamond's TPS­1 (4) complete filter sterilized medium according to the method of Visvesvara (14).

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